Screening of Active Ingredients in Rehmannia glutinosa and Its Mechanism of Action in the Treatment of Liver Diseases

Rehmannia glutinosa extracts have profound medicinal value in the treatment of liver diseases. It contains micro ribonucleic acid molecules that an average 21 nucleotides. These acids are responsible for gene regulation and expression during The study's main objective is to screen active ingredients its mechanism action treating Our study used omnibus database obtain datasets associated with GSE167308 dataset consisted 19 samples profiles dissected hepatocytes from human 3 groups (alcoholic hepatitis (n=7), alcoholic cirrhosis (n=7) controls (n=5)). GSE54350 involving CD14+ cells diabetic non-diabetic samples. We GEO2R tool, ShinyGO, R software, STRING, Network Analyst tools analyze differentially expressed genes. obtained 25 significantly genes adjusted p-value 0.05 log(Fold Change) -1.0. Also, we seven glutinosa, their descriptions target proteins. were enriched various Kyoto Encyclopedia Genes Genomes pathways such as nuclear factor-κB, Mitogen-activated protein kinases, glycolysis, Protein kinase B-serine/threonine 1 signaling, autophagy, or Transforming growth factor-beta signaling pathways. protein-protein interaction networks revealed 9 hub (Interleukin 6, Superoxide dismutase 1, Catalase, tumor necrosis factor, toll-like receptor 4, Proliferator-Activated Receptor Gamma, Nuclear Factor Kappa B Subunit NOD-Like family Pyrin domain containing prostaglandin-endoperoxide synthase 2) significant exerts anti-inflammatory response through catalpol, Rehmannioside A, B, C, Shanzhiside. mainly iridoid glycosides activates factor kappa mitogen-activated reduces production pro-inflammatory cytokines. several components (for example, Catalpol, A Shanzhiside) crucial regulating cytokines